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Name  
    (2006-09-23 10:44:18, Hit : 3284, Vote : 0)
Link #1  
   http://www.piercenet.com/Proteomics/browse.cfm?fldID=21518847-2D72-475F-A5B9-B236EC5B641E
Subject  
   stacking gel
To obtain optimal resolution of proteins, a “stacking” gel is poured over the top of the “resolving” gel. The stacking gel has a lower concentration of acrylamide (larger pore size), lower pH and a different ionic content. This allows the proteins in a lane to be concentrated into a tight band before entering the running or resolving gel and produces a gel with tighter or better separated protein bands.



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