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| Archives 2006 ³â 11 ¿ùÀÇ ±Û¸ñ·Ï |
| [2006/11/28] |
| [2006/11/23] |
| [2006/11/11] |
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¹Ì±¹ ½Ã»çÁÖ°£Áö 'À¯¿¡½º´º½º¾Øµå¿ùµå¸®Æ÷Æ®'°¡ Çظ¶´Ù ¹ßÇ¥ÇÏ´Â ¹Ì±¹ ´ëÇпø ¼øÀ§¿¡¼ Áö³ÇØ¿¡ ÀÌ¾î ¿ÃÇصµ ÇϹöµå ´ëÇÐÀÌ 1À§¸¦ Â÷ÁöÇß´Ù. Ķ¸®Æ÷´Ï¾Æ¿¡¼´Â ½ºÅÙÆ÷µå 2À§, ¹öŬ¸® 6À§, UCLA 11À§, USC 26À§, UC Davis 42À§, UC Irvine.ÀÌ 49À§¸¦ Â÷ÁöÇß´Ù.
1. Harvard University (MA)
2. Stanford University (CA)
2. University of Pennsylvania (Wharton)
4. Massachusetts Institute of Technology (Sloan)
4. Northwestern University (Kellogg) (IL)
6. Dartmouth College (Tuck) (NH)
6. University of California Berkeley (Haas)
8. University of Chicago
9. Columbia University (NY)
10. University of Michigan Ann Arbor (Ross)
11. Duke University (Fuqua) (NC)
11. University of California?Los Angeles (Anderson)
13. New York University (Stern)
14. University of Virginia (Darden)
15. Cornell University (Johnson) (NY)
15. Yale University (CT)
17. Carnegie Mellon University (Tepper) (PA)
18. Emory University (Goizueta) (GA)
18. University of Texas Austin (McCombs)
18. University of Washington
21. Ohio State University (Fisher)
21. University of North Carolina Chapel Hill (Kenan-Flagler)
23. Purdue University West Lafayette (Krannert) (IN)
23. University of Minnesota Twin Cities (Carlson)
23. University of Rochester (Simon) (NY)
26. University of Southern California (Marshall)
27. Georgetown University (McDonough) (DC)
27. Indiana University Bloomington (Kelley)
27. University of Illinois Urbana-Champaign
27. University of Maryland College Park (Smith)
31. Arizona State University Main Campus (W. P. Carey)
32. Georgia Institute of Technology
32. Michigan State University (Broad)
32. Texas A&M University College Station (Mays)
32. University of Notre Dame (Mendoza) (IN)
32. Washington University in St. Louis (Olin)
37. Pennsylvania State University University Park (Smeal)
37. University of Iowa (Tippie)
37. University of Wisconsin?Madison
40. Brigham Young University (Marriott) (UT)
40. University of Arizona (Eller)
42. University of California Davis
42. University of Florida (Warrington)
42. Wake Forest University (Babcock) (NC)
45. Tulane University (Freeman) (LA)
45. University of Georgia (Terry)
45. Vanderbilt University (Owen) (TN)
48. Boston University
49. Rice University (Jones) (TX)
49. University of California Irvine
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¡Ü Finnigan LTQ Áú·® ºÐ¼®±â
Áú·®ºÐ¼®±â´Â »ý¸í°úÇÐ, ƯÈ÷ »ýüºÐÀÚ ¹× ´ë»çü¸¦ ºÐ¼®Çϴµ¥ ÀÖ¾î °¡Àå ½Å·Ú¼º ÀÖ´Â °á°ú¸¦ º¸¿© ÁÖ´Â ºÐ¼®ÀåºñÀÌ´Ù. ÃÖ±Ù ¿¬±¸°¡ È°¹ßÇÑ Proteomics(´Ü¹éÁúüÇÐ) ¿¬±¸¿Í °ü·Ã, ESI(Electro Spray Ionization) ±â¼úÀÇ ¹ßÀü°ú ÇÔ²², MDLC(multi dimensional liquid chromatography) µîÀÇ ºÐ¸®±â¼ú ¹× ion trap MS¡¤MS¿Í °°Àº Áú·®ºÐ¼®±â¼úÀÇ ¹ßÀüÀº º¸´Ù º¹ÀâÇÏ°í ³·Àº³óµµÀÇ ´Ü¹éÁú½Ã·á¿¡ ´ëÇÑ ºÐ¼®À» °¡´ÉÄÉ ÇÏ°í ÀÖ´Ù.
Â÷¼¼´ë Áú·®ºÐ¼®±â¼úÀº ±âÁ¸ÀÇ 2D GEL ¹× MALDI-TOF ºÐ¼®±â¼úÀÇ ÇѰ踦 ±Øº¹ÇÏ°í, »ó´ëÀûÀ¸·Î ³ÐÀº ³óµµ¹üÀ§ÀÇ ´Ü¹éÁú¡¤ÆéƼµå¸¦ high-throughputÀ¸·Î È®ÀÎÇÔ°ú µ¿½Ã¿¡ ±× ¿°±â¼¿À» ºÐ¼®Çϸç, Àü»çÈļö½ÄÈ(PTM, post translational modification)µÈ ÆéƼµå ºÐ¼®ÀÌ °¡´ÉÇØ È°¿ëÀÌ ±Þ¼ÓÇÏ°Ô Áõ°¡ÇÏ°í ÀÖ´Â °æÇâÀ» ³ªÅ¸³»°í ÀÖ´Ù.
Â÷¼¼´ë ÀÌ¿ÂÆ÷Áý Áú·®ºÐ¼®±â·Î ºÒ¸®´Â Finnigan LTQ´Â HPLC¿¡¼ ºÐ¸®µÇ¾î ³ª¿À´Â »ýü¹°ÁúµéÀ» on-lineÀ¸·Î ±âüÀÌ¿ÂÈ ½ÃŲ ÈÄ, ¸ñÀû¹°ÁúÀ» ÀÏÁ¤ÇÑ °ø°£ ¾È¿¡ Æ÷ÁýÇÏ°í ºÒÈ°¼º ±âü¿Í Ãæµ¹½ÃÄÑ ³ª¿À´Â °íÀ¯ÀÇ µþºÐÀÚµéÀ» ÀÌ¿ëÇÏ¿© ±× ±¸¼ººÐÀÚÀÇ ¼¿À» ÀÚµ¿À¸·Î È®ÀÎÇÏ¿© ÁÖ´Â ÀåºñÀÌ´Ù.
±âÁ¸Àåºñ¿¡ ºñÇØ Æ÷ÁýÈ¿À²°ú Æ÷Áý·®À» ´ëÆø Áõ°¡½ÃŲ ¼±Çü hyperbolic ion trapÀ» žÀçÇÏ°í µÎ°³ÀÇ °ËÃâ±â¸¦ »ç¿ëÇÏ¿© °¨µµ, °ËÃâ¼Óµµ, Á¤·®¼ºÀ» µ¿½Ã¿¡ ³ô¿´±â ¶§¹®¿¡ È¥Çչ̷®½Ã·áÀÇ °íÈ¿À² ÀÚµ¿ºÐ¼®¿¡ Å©°Ô ±â¿©ÇÏ°í ÀÖ´Ù.
LTQ´Â ¼¼°èÀûÀ¸·Î ±× ¼º´ÉÀÌ ÀÔÁõµÈ LCQ Ion Trap Áú·®ºÐ¼®±â¸¦ ±Ù°£À¸·Î Áøº¸µÈ ±â¼úÀÌ ÁýÀûµÈ Linear Ion TrapÀ¸·Î¼, º¸´Ù Á¤±³ÇØÁø ÀÌ¿ÂÈ ±â¼ú·Î ¿ë¸ÅÁ¦°Å¸¦ ÃÖÀûȽÃŲ »õ·Î¿î Ion Max Source°¡ ÀåÂøµÆÀ¸¸ç, ¹æ»çÇüÀÇ À̿¹èÃâ±â¼ú°ú ÇÔ²² µÎ°³ÀÇ ÀüÀÚÁõÆø °ËÃâ±â¸¦ žÀçÇÏ¿© atto-mole ¼öÁØÀÇ ³óµµ¿¡ ´ëÇÑ °ËÃâÀ» °¡´ÉÇÏ°Ô ÇÑ´Ù.
¼¼ ±¸¿ªÀ¸·Î ³ª´©¾îÁø Linear ion trapÀº ±âÁ¸ Àåºñµé¿¡ ºñÇØ 10¹è ÀÌ»óÀÇ °¨µµÇâ»óÀ» °¡Á®¿ÔÀ¸¸ç, »ç¿ëÀÚ°¡ ÇÊ¿ä·Î ÇÏ´Â ¸ðµç MS?MSn ±â´ÉÀ» ºü¸¥ ¼Óµµ·Î Á¦°øÇÔÀ¸·Î½á º¸´Ù Á¤È®ÇÏ°í ºü¸¥ ºÐ¼®À» °¡´ÉÇÏ°Ô ÇÑ´Ù.
¿¹¸¦ µé¾î 2D-LC ¿Í º´ÇàÇÏ¿© sub femtomole ~ nanomole ³óµµ¹üÀ§ÀÇ ´Ü¹éÁúÈ¥Çս÷Ḧ µ¿½Ã¿¡ ºÐ¼®ÇѴٰųª, 1ÃÊ¿¡ 3¹ø ÀÌ»óÀÇ MS/MS¸¦ ÀÚµ¿À¸·Î ¼öÇàÇÔÀ¸·Î½á LC¿¡¼ ¹Ìó ºÐ¸®°¡ µÇÁö ¸øÇÏ°í co-elutionµÇ´Â ÆéƼµåµé¿¡ ´ëÇؼµµ ³õÄ¡Áö ¾Ê°í ±× ¼¿Á¤º¸¸¦ Á¤È®ÇÏ°Ô ÁÙ ¼ö ÀÖ´Â ±â´É µîÀÌ ¹Ù·Î ±×°ÍÀÌ´Ù. ºÐ¸®±â¼úÀÇ ¹ß´Þ°ú ÇÔ²² LTQÀÇ ÀÌ·¯ÇÑ ½Å°³³ä LC-MS/MS ±â¼úÀ» ÅëÇØ, ±âÁ¸ÀÇ ±â¼ú·Î´Â ½±°Ô ºÐ¼®ÇÏÁö ¸øÇß´ø PTM ´Ü¹éÁúÀ̳ª ¹ÙÀÌ¿À¸¶Ä¿(biomarker, »ýüǥÁö¹°Áú)¸¦ º¸´Ù ºü¸£°í ½±°Ô È®ÀÎÇÒ ¼ö ÀÖ°Ô µÇ¾úÀ¸¸ç ±× È°¿ë¿¹°¡ ÃÖ±Ùµé¾î È°¹ßÈ÷ ¹ßÇ¥µÇ°í ÀÖ´Ù.
¶ÇÇÑ LTQ¿Í FT-ICR Áú·®ºÐ¼®±â°¡ °áÇÕµÈ LTQ-FT¸¦ ÀÌ¿ëÇϸé top-down Proteomics ¹× °íºÐÇØ¡¤°íÁ¤È® ¿°±â¼¿ºÐ¼®ÀÌ °¡´ÉÇѵ¥, ÀÌ·¯ÇÑ ºÐ¼®±â¼ú ¿ª½Ã ÃÖ±Ù Àü¼¼°èÀûÀ¸·Î Å« ¹ÝÇâÀ» ºÒ·¯ÀÏÀ¸Å°°í ÀÖÀ¸¸ç °í°¡ÀÇ ÀåºñÀÓ¿¡µµ ºÒ±¸ÇÏ°í ÇâÈÄ ±× º¸±Þ ¹× È°¿ëÀÌ ±Þ°ÝÈ÷ ´Ã¾î³¯ °ÍÀ¸·Î Àü¸ÁµÈ´Ù.
MALDI ¹× ESI ¿Í °°ÀÌ »ýüºÐÀÚ¸¦ ±âüÀÌ¿ÂÈ ÇÒ ¼ö ÀÖ´Â ±â¼úµéÀÌ °³¹ßµÈ ÀÌÈÄ Áú·®ºÐ¼®±â¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú ºÐ¼®±â¼úÀº ÇÏ·ç°¡ ´Ù¸£°Ô ¹ßÀüÇÏ¿© ÇöÀç´Â ´Ü¹éÁú ¿¬±¸ºÐ¾ß¿¡¼ °¡Àå ¹ÏÀ»¸¸ÇÑ ºÐ¼®±â¼ú·Î ÀÚ¸®Àâ¾Ò´Ù.
Finnigan LTQÀÇ ÀÀ¿ëºÐ¾ß¸¦ »ìÆ캸¸é ¡â°íÈ¿À² ´Ü¹éÁú È®ÀÎ(high throughput protein identification) ¡â¾ÆÁ÷±îÁö ¹àÇô³»Áö ¸øÇÑ ¼ö¸¹Àº ¹ÙÀÌ¿À¸¶Ä¿¸¦ ã¾Æ³»°í Á¤·®ÈÇÏ´Â ´Ü¹éÁúÀÇ Á¤·®ºÐ¼® ¡âMALDI-LTQ ¹× LTQ-FT/MSµîÀÇ hybrid Áú·®ºÐ¼® ¡âÀü»çÈļö½ÄÈ(PTM, post translational modification)µÈ ´Ü¹éÁúÀÇ ÀÚµ¿È ºÐ¼® µîÀ» ²ÅÀ» ¼ö ÀÖ´Ù.
¡Ü ÁÖ¿ä »ç¾ç
Mass Analyzer : Three segmented Linear Ion trap
Detector : Dual EMs with +/-15KV
Mass Range : 15 ~ 4,000 (m/z)
Mass Resolution : R=20,000 (FWHM) with ZoomScan
Scan Cycle Time : 5 spectra/sec
MS/MSn Function : n= 10, automated
MS/MS Sensitivity(S/N) : S/N 100:1 @ 250 fg Reserpine
(2006 . 6 񃯇)
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[ÇÑ»ïÈñÀÇ È¯°æÄ®·³] ºÎ²ô·¯¿î ¹Ù´Ù À̾߱â
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